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DABCYL-gamma-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS CAS NO.127134-13-8
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Keywords
- DABCYL-γ-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS
Quick Details
- ProName: DABCYL-gamma-Abu-Ser-Gln-Asn-Tyr-Pro-I...
- CasNo: 127134-13-8
- Molecular Formula: C73H97N17O18S
- Appearance: Red Powder
- Application: For research use only.
- Purity: 95%
- Storage: Store at -20°C
- LimitNum: 0
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Details
DABCYL-gamma-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS, the fluorogenic HIV-1 protease substrate, consists of an octapeptide with a fluorescent donor (EDANS) and a quenching acceptor (DABCYL), linked to the COOH- and NH2-terminal. The γ-abu spacer is inserted to avoid the potential steric hindrance of substrate binding by bulky acceptor. The FRET substrate is cleaved by the HIV-1 protease on the Tyr-Pro bond, resulting in increased fluorescence intensity in a time-dependent (excitation at 340 nm, emission at 490 nm).
